📝 Quick Start & Notes
1. Input protein structure
- Upload a PDB file or enter a PDB ID / UniProt ID.
- Structures are automatically retrieved from RCSB PDB or AlphaFold DB.
2. Structure requirements
- Only single-chain proteins are currently supported.
- Multi-chain complexes and protein assemblies are not supported in the public server version.
3. Finetuning option
- Enable finetuning to perform lightweight meta-transfer learning for protein-specific adaptation.
- Typical finetuning takes ~10 min depending on server workload.
⚠️ Warning:
Fine-tuning of proteins larger than 800 residues is not recommended on the public server due to computational resource limitations.
Fine-tuning of proteins larger than 800 residues is not recommended on the public server due to computational resource limitations.
For longer proteins, please use recommended alternatives:
Colab notebook
·
Local software from GitHub
4. Output results
- high-fitness mutations
- interactive protein structure visualization
- mutation fitness landscape analysis
💡 What can unZipro do?
- Enzyme engineering
- Genome editing tools (SpCas9, Cas12, base/prime editors)
- Plant protein engineering (virus resistance, transcription factors)
- Protein therapeutics
- General protein design in biotechnology & agriculture
🧪 Experimentally validated examples
| Category | Description |
|---|---|
| Genome editors | Adenine base editor (TadA8e) |
| Genome editors | Three nucleases (SpCas9, CasΦ2/Cas12j2, T5E) |
| Polymerase | MMLV reverse transcriptase under multiple conformations |
| Fluorescent enzyme | Luciferase for improved fluorescence intensity |
| Plant proteins | DNA-binding domains of plant transcription factors |
| Plant proteins | Plant virus-resistance (R) proteins |
📖 References
Please cite unZipro if used in research.