📝 How to use
- Upload your PDB file or enter a PDB ID / UniProt ID. Automatically fetches from RCSB PDB or AlphaFold DB.
- Single-chain proteins only; multi-chain complexes are not supported.
- Enable finetuning for meta-transfer learning. (~30 min typically; longer if the server is busy. Large proteins may cause memory issues during finetuning.)
- Outputs are highest-fitness mutations with interactive structure & fitness landscape visualization.
💡 What can unZipro do?
- Enzyme engineering
- Genome editing tools (SpCas9, Cas12, base/prime editors)
- Plant protein engineering (virus resistance, transcription factors)
- Protein therapeutics
- General protein design in biotechnology & agriculture
🧪 Experimentally validated examples
| Category | Description |
|---|---|
| Genome editors | Adenine base editor (TadA8e) |
| Genome editors | Three nucleases (SpCas9, CasΦ2/Cas12j2, T5E) |
| Polymerase | MMLV reverse transcriptase under multiple conformations |
| Fluorescent enzyme | Luciferase for improved fluorescence intensity |
| Plant proteins | DNA-binding domains of plant transcription factors |
| Plant proteins | Plant virus-resistance (R) proteins |
📖 References
Please cite unZipro if used in research.